The translation of messenger RNA (mRNA) into protein is an essential process for all forms of life. The ability to monitor this process in a quantitative way by ribosome profiling-based approaches has revolutionized our ability to monitor protein synthesis in vivo and to explore and model complex...
The drawback of SAGE is that the size of the sequencing effort required to generate representative data sets limits the practical applicability of the method, particularly when transcripts expressed at low levels need to be covered. mRNA differential display (DDRT-PCR) [4], cDNA-AFLP [5] and ...
Fig. 1: Enzymatic conversion of DNA microarrays to RNA. aThe three-step process of conversion consists of 1- primer hybridization followed by crosslinking, 2- T7 RNA polymerase-mediated RNA extension, and 3- DNase-mediated degradation of the DNA template.bApplication of the DNA-to-RNA method...
Industrial biotechnology heavily relies on the microbial conversion of carbohydrate substrates derived from sugar- or starch-rich crops. This dependency poses significant challenges in the face of a rising population and food scarcity. Consequently, expl
Prices may be subject to local taxes which are calculated during checkout Additional access options: Log in Learn about institutional subscriptions Read our FAQs Contact customer support Data availability The summary data of the BDNF mRNA expression in both ASD and healthy control postmortem brain samp...
[1], its hydrophobic nature contributes significantly to the stabilization of proteins’ structure [2,3]. Methionine also plays an essential role in initiating mRNA translation and indirectly regulates various cellular processes by serving as the precursor ofS-adenosyl-methionine (SAM), a biological ...
Modifications in the chemistry of mRNA can enhance stability, reduce immunogenicity, and thus facilitate mRNA‐based nucleic acid therapy, which eliminates risk of insertional mutagenesis. In addition to these valuable advantages, the mRNA‐based method showed significantly higher efficacy for reprogramming...
Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal ...
making it difficult to obtain enough sample for further analysis. See. S. J. Clark et al.Nucleic Acid Research2001, 29 no. 13, e65. Given the importance of assessment of DNA methylation, it can be seen that there is a need for improved methodologies for conversion of cytosine to uracil....
21. A double-stranded nucleic acid structure comprising two parallel Hoogsteen base-paired strands having binding affinity for a single-stranded target in a mRNA sequence or one strand of a DNA sequence; wherein said double-stranded nucleic acid structure is capable of binding through Watson-Crick...