Conventional PCR (cPCR) and real‐time quantitative PCR (qPCR) are two widely used approaches employed in environmental DNA (eDNA) detection, though lack of a comprehensive comparison of them impedes method selection. Here we test detection capacity and false negative rate of both approaches using ...
相比于常规PCR ,实时PCR测定需要较少HANDSON 翻译结果2复制译文编辑译文朗读译文返回顶部 与传统的 PCR 相比,实时的 PCR 分析需要更少 handson 翻译结果3复制译文编辑译文朗读译文返回顶部 实时pcr 与常规 PCR 相比,需要更少的牵手 翻译结果4复制译文编辑译文朗读译文返回顶部 ...
meat bone meal (MBM).Here,A TaqMan technology of Real-time PCR and a conventional PCR were developed to detect the bovine material in the MBM.Template DNA of bovine was extracted from MBM using Chelex-100,and this step spent only about 20 minutes.The conventional PCR could detect the 0.001...
clinical samplessensitivityreal-time PCRWSSVWSD.The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed...
A real-time PCR method has been develo... CM Cheng,W Lin,KT Van,... - 《J Food Prot》 被引量: 225发表: 2008年 Evaluation of TaqMan PCR Assay for Detecting Salmonella in Raw Meat and Shrimp We evaluated the TaqMan Salmonella amplification/detection kit from PE Applied Biosystems, which...
Real-time PCR (TaqMan) assays were developed for the specific detection and discrimination of Colletotrichum spp., C. acutatum and C. gloeosporioides causi... C Garrido,M Carbú,F. J. Fernández‐Acero,... - 《Plant Pathology》 被引量: 43发表: 2010年 A Multiplex PCR Method for the Ident...
peopleintheportlaboratoriestoidentifythesoybeangermplasmresources. KeywordsSoybeangermplasmresources;UNK2gene;ConventionalPCR;Real-timePCR 摘要根据一种新的大豆内源特异性基因(UNK2)序列设计合成引物和探针,建立了一种基于 该基因的普通PCR和实时荧光PCR检测方法。结果显示,该方法在普通PCR和实时荧光PCR检测 中均具有...
The inexpensive, sensitive and rapid real-time PCR based in SYBR Green method is of interest in monitoring Legionella pneumophila contamination, especially in environmental samples, and should be economical for large-scale routine tests. 展开 关键词: legionella pneumophila real time pcr sybr green ...
Samples of rice from Mexican and USA retail stores were analyzed for the presence of transgenic (GM) events using real-time PCR. In screening for the CaMV3
COMPARISON OF CONVENTIONAL AND REAL TIME PCR METHODS TOD, Determine T H E A C E IAlp, EbruMenevşe, Sevda