Subsequently, slices were incubated for 2 h in biotinilated antibody goat anti-rabbit (1:200, Vector labs) in 3% NGS in PBS, and afterwards in Biotin/Avidin (1:1000, Vectastain) in PBS for 1 h. This complex was visualized by exposing the slices for 5 min to a solution of ...
Summary The use of aircraft to control important vector borne disease outbreaks through the selective application of pesticides continues to play a vital role in preventing the spread of disease in many parts of the world. The deployment... JS Clayton,TPY Sander - 《Aspects of Applied Biology》...
When solving the SE problem, SVC is modeled by a susceptance variable at node i of the SVC installation, and either conductance bSVC, or angle α is involved in the state vector x instead of Ui that is fixed. In both cases, one should calculate derivatives by these variables from measuring...
At this point, the variance of a parameter vector was assessed in each condition to discard conditions with zero variance from further analysis. A single parameter at a time was added to the subject-level model, to avoid orthogonalization effects. At the group level, we used the parametrically...
(Vector Laboratories) and 3,3′-diaminobenzidine (DAB; Dako) as chromogen. Finally, tissue sections were counterstained with hematoxylin. Mouse paraffin sections (3 μm thick) were stained with haematoxylin and eosin (H&E) for histological evaluation. For immunohistochemical analysis, deparaffin...
where M= Mass Matrix (Kg); X= Displacement vector; C= Damping Matrix (N.s/m); K= Stiffness Matrix (N/m); X¨g= One-dimensional acceleration (m/s2); J= Matrix of ones; φ= Control force position matrix; F= Control force vector (N). However, the state-space representation of ge...
Machine learning phase (MLP)The gait phase can also be estimated using machine learning, with techniques such as support vector machine (SVM) or neural networks. The machine learning methods are diverse and complex, so they will not be explained here. All the found references used a different ...
filter. Based on offline analysis, only signals from 9 electrodes on the mid-line central region, corresponding to the locations FC1, FCz, FC2, C1, Cz, C2, CP1, CPz, and CP2, were selected. These 9 filtered channels are concatenated to create a 1,386-element feature vector for ...
(p15A-kanR vector). A final concentration of 1 μM aTc43was used to activate dCas9 expression from the genome, and 0.4 μM Cuminic acid was used to stably maintain TULIP at a basal copy number. At the overnight pre-culture step, appropriate concentrations of Van or AHL were ...
To monitor RAC1 activity after transient overexpression of CRK (pIRESII-EGFP-CRK vector) in C2 cells the Active RAC1 Pull-Down and Detection Kit (ThermoFisher #16118) was used according to the manufacturer’s protocol. The kit is based on a GST-fusion protein containing the PBD (p21-bindin...