With these results, PACE gives us a sense of the types of questions in basic evolutionary biology that continuous evolution can address, questions that would be difficult to study with traditional directed evolution techniques. Although these examples focus only on T7 RNAP, PACE is generalizable ...
As our null hypothesis proposed, the differential performance between nonsynonymous and synonymous SNPs might serve as evidence indicating that our observation is not merely random, and natural selection is likely to participate in this process to maintain the frequency spectrum of nonsynonymous sites. Th...
using all 8 TIDA boronates (1, 14-20) and amines (45-68) as a representative example, NL-ADE-MS allowed reaction conditions to be prioritized for specific amines directly from ultra-high throughput experimentation (representative example shown in panel m, for other examples see Extended Tabular...
using all 8 TIDA boronates (1, 14-20) and amines (45-68) as a representative example, NL-ADE-MS allowed reaction conditions to be prioritized for specific amines directly from ultra-high throughput experimentation (representative example shown in panel m, for other examples see Extended Tabular...
As expected from inter-individual variation, the prevalence distribution of species in CHILD samples shows that the majority were “private” species found in one or a few individuals, while a minority were “common” species to many individuals (Fig. 3e). The NS0 and NS1 isolate libraries ...
In the future, we aim to leverage the scalability of OrthoRep, by starting evolution from hundreds of neutral variants of PfDHFR, to capture the scope of trajectories that may be available from standing variation in natural P. falciparum populations and predict selection conditions that may prefer...
A total of 12 differential metabolites were compared between TW/CW, TB/CB, and TR/CR, with content values > 5 as the screening condition (Fig.2b). The results showed significant variation in the levels of delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, fzelin, kaempferol-3-O-...
Within MIC1, there are several examples of genes that share the same domain at the same position in the protein. This is particularly apparent in the Triticeae. Among such examples are NLR-GRAS, NLR-kinase, and NLR-NPR1 (Fig.5). This conservation in architecture indicates a common ancestry...
With this method, the same cells were analysed on each time point of the experiment to exclude variation between samples as a source of error. All results were normalised to the first day values of the control group cultured in darkness. Measurement of glutathione levels Reduced levels of ...
Due to a variation of parameters h and g, the boundary stable equilibrium \({\overline{E}}\) crosses border \(b_1\), becomes the interior equilibrium E, still remaining a fixed point of map T. Depending on the adjustment speed values, E may either remain stable or it may become ...