(CLSM; Olympus FluoView FV1200, Olympus, Tokyo, Japan). Three microscopic confocal volumes from random areas upon the dentin/material interface where the biofilm membranes were previously positioned were acquired from each sample using a 60× water immersion lens, 1 μm step-size and a format ...
Optical images of the stainless-steel plates and the load-carrying surfaces of the polymer pins before and after the reciprocating tests were obtained using a digital camera Nikon D90 (Nikon, Tokyo, Japan) equipped with a Nikon AF-s 60/2,8 G ED Macro lens (Nikon, Tokyo, Japan). After ...