--chr- when reading the reference files, the program will limit the analysis to those SNPs on this chromosome. --top_snp- maximum number of SNPs that may be selected by the stepwise selection process, default is 1e10, i.e. a lot. ...
GCTA: a tool for genome-wide complex trait analysis. Am J Hum Genet. 2011;88(1):76–82. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. http-equiv="content-type" ...
We present an approximate conditional and joint association analysis that can use summary-level statistics from a meta-analysis of genome-wide association studies (GWAS) and estimated linkage disequilibrium (LD) from a reference sample with individual-level genotype data. Using this method, we analyzed...
Real-time PCR analysis was performed in 25 μl reactions using Power SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) as recommended by the manufacturer (2.5 μl of a 20×-diluted RT-reaction as template, 0.2 μM of each primer). UBC (ON56/ON57) and PPIA (ON174/ON175...
Although this has been reported in other studies,11 random insertion analysis of the Scyl1AIv4 cassette using primers within the HDR template failed to reveal insertion in F1 mice other than the ones identified as having the proper insertion.1 Although random integration of HDR template remains ...
Study of Interaction between Hha and TomB. Analysis of the interaction between Hha and TomB was performed by a previously described protocol42. Briefly, cell lysates expressing chromosomally His-tagged Hha and TomB were allowed to bind to Ni2+NTA beads for precipitation of His tagged protein....
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An essential step of this analysis is a conditional analysis. It aims to eliminate the influence of SNPs outside the gene, which are in linkage disequilibrium with intragenic SNPs. The popular conditional analysis method, GCTA-COJO, accounts for the influence of several top independently associated...
(d) Immunoblot analysis of Bcl-2 and Bax in retinas of HMGB1Δrod mice and littermate controls at 4 h and 3 d post RD. (e) Graphs show the quantification of protein levels based on densitometry of the Western blots in (d). Naïve retinas without manipulation of either eye was used ...