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Each condensin complex is composed of two ATPase subunit heterodimers (structural maintenance of chromosomes (SMC) 2 and 4) and three non-SMC regulatory subunits2,3. A unique set of three non-SMC regulatory components defines each condensin complex; NCAP-D2, NCAP-G and NCAP-H are parts of...
Although sharing the same SMC subunits (SMC2 and SMC4), condensin I and II have distinct non-SMC regulatory subunits, including the kleisin subunit (CAP-H and CAP-H2, respectively) and a pair of HEAT repeat subunits (CAP-D2/G and CAP-D3/G2, respectively; Figure 1B). Though the ...
Using our mRNA display technology, we identified hCAP-G2, a subunit of condensin II complex, which is regarded as a key player in mitotic chromosome condensation, as a Q15 binding partner. Immunofluorescence study indicated that Q15 compromises normal segregation of chromosomes, and therefore ...
(b) Suppressor mutations in three independent phenotypic revertants ofcut3were revealed by whole-genome sequencing to reside inmed6(Mediator complex subunit 6). X, nonsense mutation; fs, frameshift mutation. (c) Rescue of growth defect in condensin mutants bymed6deletion (med6Δ). Med6 ...
we identified hCAP-G2, a subunit of condensin II complex, which is regarded as a key player in mitotic chromosome condensation, as a Q15 binding partner. Immunofluorescence study indicated that Q15 compromises normal segregation of chromosomes, and therefore might induce apoptosis. Thus, our resul...
To test the role of condensin in dicentric centromere relocation, we created a conditional 6+7 dicentric in cells carrying an auxin-inducible degron of Smc2, a SMC subunit of yeast condensin (Figures S4C and S4D). Download: Download high-res image (832KB) Download: Download full-size ...
& Felsenfeld, G. Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activity. Mol. Cell. Biol. 31, 2174–2183 (2011). CAS PubMed PubMed Central Google Scholar Haeusler, R.A., Pratt-Hyatt, M...
(YGRC) for fission yeast strains. We also thank L. Showe, P. Lieberman and R. Locke for critically reading the manuscript, and S. Shaffer for editorial assistance. This work was supported by the G. Harold and Leila Y. Mathers Charitable Foundation and the NIH Director's New Innovator ...
sequences, with B24 being derived from B237,38. Likewise, we analyze the transcriptional activity of theB-CAP-Gpseudogene and its possible influence on the activity of other condensin complex protein subunit genes (CAP-D2andCAP-D3), as an indirect indication for its possible functional role....