An equal volume of 2× SDS gel-loading buffer (90 mM Tris/HCl pH 6.8, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue, 20% (v/v) glycerol, and 10% (v/v) 2-mercaptoethanol) was added, and the samples were heated at 100 °C for 10 min to terminate the PK reaction. SDS-...