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We tested if this distal TATA box is required for TBX18 transcription by precisely deleting 40 bp surrounding it using CRISPR-Cas9-homology-directed repair (HDR) (Figure 4B). Two independent, pluripotent EBdCas9 cell lines with homozygous TATA box deletions were generated, TATA Δ #1 and TATA ...