After that, cells were freeze-thawed twice by using liquid nitrogen and a heating block set at 25 °C in order to ensure a uniform temperature between tubes after keeping at −80 °C overnight. 50 μL of supernatant were collected by centrifugation (eppendorf centrifuge 5415R, 13,...
Fear conditioning measures hippocampal-dependent associative learning. The read-out measured is a freezing response where the mouse will freeze if it remembers and associates that environment with the aversive stimulus. The hippocampus and the amygdala are required for fear memory [18,19]. Contextual ...