Cells were fixed in 2.5% glutaraldehyde, 3% paraformaldehyde with 5% sucrose in 0.1 M sodium cacodylate buffer (pH 7.4), pelleted, and post fixed in 1% OsO4 in veronal-acetate buffer. The cells were stained en bloc overnight with 0.5% uranyl acetate in veronal-acetate buffer (pH 6.0...
The DNA was precipitated by adding 1/10 volume of sodium acetate (3 M, pH 5.0) and 2.5 volumes of ethanol (95%) and resuspended in 30 μl Milli-Q water. As a negative control, a blank sample containing 5 ml Milli-Q water instead of 5 g of soil was processed as described above....
After sample loading, stage tips were washed with 1% FA and phosphate/citrate buffer (0.23 M sodium phosphate and 86.4 mM citric acid [pH 5.5]). At this point, the “light” solution (0.4% CH2O and 60 mM NaBH3CN), or “heavy” solution (0.4% CD2O and 60 mM NaBD3CN)...
The present invention relates to a novel adalimumab liquid pharmaceutical composition comprising adalimumab or a biosimulator thereof, a citrate buffer / system such as sodium citrate / citric acid, and a sugar stabilizer such as trehalose . Combination of such ingredients results in formulations with...
Onozuka R-10 (Serva), 0.7 % cellulysin cellulase (Calbiochem), 0.7 % cytohelicase (Sigma), 1 % hemicellulase (Sigma), 4 % pecti- nase (Sigma), 20 % pectolyase (Sigma) in enzyme buffer (4 mM citric acid, 6 mM sodium citrate in ddH2O, pH 4.5) for 60 – 90 min at 37 °C...
The optical density (OD) of an aliquot was measured at 620 nm, based on the relationship CFU/mL = OD620 × 2.5.108; a volume containing 4 × 106bacterial CFU was added to 10 ml of underlay gel (10 mM sodium phosphate buffer, 0.3 mg/ml TSB powder, and 1% ...
Thereafter, microwaving was carried out for 3 times 5 min in 10 mM sodium citrate buffer at pH 6 to allow the accessibility of epitopes. Subsequently, the samples were allowed to cool and treated with a series of washing steps (once in dH2O and 3 times 5 min in PBS). Non-specific ...
The cellular composition of barrier epithelia is essential to organismal homoeostasis. In particular, within the small intestine, adult stem cells establish tissue cellularity, and may provide a means to control the abundance and quality of specialized epithelial cells. Yet, methods for the identificati...
sodium hydroxide, glycine, hydrochloric acid, citric acid, etc., local anesthetics represented by benzyl alcohol, isotonizing agents such as sodium chloride, mannitol, sorbitol, etc., adsorption inhibitors such as Tween 80 etc., solubilizers such as cyclodextrins and derivatives thereof, stabilizers ...
chelating agents such as disodium edetate and sodium citrate; agents for making isotonic solutions, such as sodium chloride, sorbitol and glycerin; buffer agents such as phosphates, boric acid and citrates; and pH controlling agents such as hydrochloric acid, acetic acid and sodium hydroxide. The ...