Under basic conditions at elevated temperatures, the column material deteriorated more quickly, but still remained stable up to 100°C at pH 9 and 60°C at pH 10. The results of this study indicate that this novel C18 silica-based column represents a significant advancement in RPLC column ...
Peptides were subjected to a 50-min linear gradient from 5 to 35% acetonitrile in 10 mM ammonium bicarbonate pH 8 at a flow rate of 0.6 ml/min over an Agilent 300Extend C18 column (3.5 μm particles, 4.6 mm ID and 220 mm in length). The peptide mixture was fractionated ...
Piper nigrum L. is a widely used spice because of its flavour and health effects. It is prepared as black and white pepper, according to the harvest time and inclusion of the outer skin. Pepper pericarp is usually considered waste when making white peppe
Peptides were trapped on a µPrecolumn C18 PepMap100 (5 μm, 100 Å, 300 μm × 5 mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm, 100 Å, 75 μm × 15 cm, C18) by applying a 60-min gradient...
The ethyl acetate extract was analyzed by HPLC on a RP-C18 column (25×4.5 mm) using methanol as solvent and UV detection at 420 nm. The eluant flow rate was 1 ml/min. Efficiency of the extraction procedure for recovering curcumin from blood samples was determined by measuring recovery of...
Afterwards, the aqueous end of the mixture was run off while the organic solvent end was collected into a 25 ml standard flask. The analysis was performed on a Shimadzu HPLC system (NexeraMX) fitted with uBONDAPAK C18 column (length 100 mm, diameter 52 (4.6 mm, and thickness 7 μm). ...
The ExionLC Infinity series UHPLC System (AB Sciex) with the Kinetex C18 column (2.1 × 100 mm, 1.7 μm; Phenomen) was used for LC/MS analyses. The mobile phase A consisted of 40% water and 60% acetonitrile with 10 mmol/L ammonium formate, and the mobile phase B consisted of...
. 200 μl of extract were generated from each T175 cm2flask. Extracts were stored at −80° C. in 50 ul aliquots. Prior to use extracts were semi-purified, removing low molecular weight interfering components by passing through a small G-25 Sephadex 50-100 μl spin-column (Roche or ...
3. The process of claim 1, wherein the heavy alkyl benzene fractions (C18-22) used is obtained from mono and di alkyl benzenes produced during the production of linear alkyl benzene (LAB) in detergent industry, heavy alkyl aromatics produced in catalytic reformer, and naphtha or gas steam ...
On the C18 column (15 μm global filler), linear elution was carried out in water/acetonitrile containing 0.1% TFA. The purity of the purified Fusonex peptide, which was measured by analytical HPLC, was higher than 95%. At last, Fusonex is verified by amino acid sequencing and electron ...