Explain DNA amplification (PCR) and give one example of when it would be used. List the functions of RNA. What is DNA polymerase? What function does it have? Describe the components and process of RT-PCR to amplify and synthesize cDNA from an mRNA . ...
PCR primers are synthetic DNA oligonucleotides of approximately 15–30 bases. PCR primers are designed to bind (via sequence complementarity) to sequences that flank the region of interest in the template DNA. During PCR, DNA polym...
During PCR, DNA polymerase extends the primers from their 3′ ends. As such, the primers’ binding sites must be unique to the vicinity of the target with minimal homology to other sequences of the input DNA to ensure specifi...
So far we have identified at least one deletion allele in each subclass and we are in the process of identifying alleles of the remaining genes. We plan to determine what other cellular components interact with these proteins to mediate their specific functions, and to discern which proteins ...
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List the three major types of RNA and describe their functions. Describe three types of DNA binding domains. Describe four nucleotides with different biochemical functions. What are the structure and chemistry of nucleotides? Name the three DNA Sequence Databases and where they are located. ...
Real-time PCR and data analysis was performed as described previously [29]. Results represent three assays performed in duplicated.Identification of proteins by liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS) HeLa or K562 nuclear extracts were immunoprecipitated with anti-MCP1 ...
Lignin is absent, or in very low concentra- tions, in the cells of fruit pulp and vegetables at their edible stages [6, 9]. Lignin affects the rigidity and cohe- sion of the walls [6]. Citrus fruit are hesperidia. The fruit are divided intern- ally into 9–14 juice vesicle-...
We have also shown that the homodimers of YAP2L/TAZ are more stable and showed more oncogenic behaviour than their corresponding monomers as revealed by colony formation and cell transformation assay. Since cysteine post-translational regulation plays important roles in redox signalling, tumorigenesis ...
During PCR, DNA polymerase extends the primers from their 3′ ends. As such, the primers’ binding sites must be unique to the vicinity of the target with minimal homology to other sequences of the input DNA to ensure specifi...