Bacterial operons containing components of the translation machinery are of great antiquitydoi:10.1007/BF02459889GeorgeDept.E.Dept.FoxDept.KirtDept.A.Dept.MartinDept.WilliamDept.R.Dept.WidgerDept.FadhiDept.SpringerOrigins of Life & Evolution of the Biosphere...
Using this strategy, five insertions in genes/operons positively regulated by OmpR and two insertions in genes negatively regulated by this protein were identified. Genetic analysis of one of these fusion strains revealed that the gene acrR, encoding transcriptional repressor AcrR is negatively ...
The F1F0 ATP synthase was encoded in a gene cluster (atp/BEFHAGDC) and the nine genes were transcribed as an operon. PhoP positively regulated the transcription of the nine ATP synthase genes and exerted this effect through direct binding to the promoter of atpl. Overall, the results provide...
The results obtained demonstrate that the apcEA1B1C gene cluster forms an operon from which segmented transcripts originate, whereas the apcA2 gene behaves as a monocistronic unit. Qualitatively, the same transcripts were identified regardless of the light wavelengths received during cell growth. The...
SNARE itself is part of an even larger complex known as the 20 S particle. The 20 S particle was identified through work on Rattus norvegicus (rat) liver tissue using purified recombinant human N-ethylmaleimide-sensitive fusion protein (NSF) and α-soluble N-ethylmaleimide-sensitive fusion ...
Reduced levels of frataxin, an essential protein of as yet unknown function, are responsible for causing the neurodegenerative pathology Friedreich's ataxia. Independent reports have linked frataxin to iron–sulphur cluster assembly through interactions
This study provides an approach for efficient translation from an operon-based system into a polyprotein-based assembly that has the potential for portable and stoichiometric expression of the complex nitrogenase system in eukaryotic organisms. 展开 关键词: nitrogen fixation polyprotein synthetic biology ...
In this study, we obtained biochemical evidence supporting the view that when KdpE is phosphorylated, it takes on an active form that exhibits relatively high affinity for the kdpABC promoter, which in turn results in activation of the kdpABC operon. It was also suggested that the central ...
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa PAO1. The operons, designated phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2, a... DV Mavrodi...
of molecules involved in upstream processes, such as the intake of inducers from the environment, acts only as a transient source of variability in RNA production numbers, while variability in the numbers of a molecular species controlling transcription of an active promoter acts as a constant ...