Here we have explored the interaction between the actin cytoskeleton and p40phox, one of the cytosolic components of NADPH oxidase. Full length p40phox expressed in COS cells co-localised with F-actin in a peripheral lamellar compartment. The co-localisation was lost after deletion of the Phox ...
In vegetative cells centrosomes generally are tightly coupled to the nuclear envelope through so-called LINC complexes (linker of nucleoskeleton and cytoskeleton) consisting of a SUN protein in the inner nuclear membrane, and a KASH-domain protein in the outer nuclear membrane (Crisp et al., ...
Retrieval of CI-MPR from endosomes to the TGN re- quires dynein/dynactin motor activity and the microtu- bule cytoskeleton Most of the retrograde vesicular transport from the cell periphery to the center requires activity of the micro- tubule-based dynein/dynactin motor complex. To deter- mine...
4,5-trisphosphate.124 In addition to cell signaling, the recruitment of proteins to specific membrane compartments via phospholipid recognition can modulate a variety of other processes including endocytosis, phagocytosis, membrane/organelle fusion, membrane– cytoskeleton adhesion and ion channel function. ...
The CIL can be searched using GO terms, and the hierarchy of the GO is incorporated into the query. For example, searching for ‘cytoskeleton’ (GO:0005856) returns images annotated directly with ‘cytoskeleton’, as well as cytoskeletal parts, such as ‘microtubule basal body’ e.g. http:...
Blood flow through the network of mucosal microvessels in the gastrointestinal tract is essential for delivery of oxygen and nutrients to all mucosal constituents. The endothelial cells lining the microvessels, although protected by prostaglandins, are m
Actin cytoskeletonCaldesmonPodosomeVascular smooth musclePodosomes are highly dynamic actin-based structures commonly found in motile and invasive cells such as macrophages, osteoclasts and vascular smooth muscle cells. Here, we have investigated the role of caldesmon, an actin-binding protein, in the ...
1998). Moreover, both LIS1 and DCX proteins regulate the microtubule cytoskeleton (49, 13, 18). LIS1 encodes a ubiquitously expressed 45 kDa protein with seven WD40 repeats (Reiner et al. 1993) and has recently been shown to be a microtubule-associated phosphoprotein that interacts with ...
Transcription of XYbp is regulated during spermatocyte differentiation. The antiserum raised against the XYbp peptide demonstrated that XYbp is localised mainly in the XY bivalent of spermatocytes (XY body) and in the centrosomes of somatic and germ cells in all phases of the cell cycle. These...
normalized to 6 h).dNumber of cells infected with WT (white) or EXP2 cKO (blue) sporozoites analyzed by immunofluorescence (>30,000 cells analyzed,N = 4 or 5 totaling 12 or 15 replicates for 2 h or other timepoints, respectively, normalized to 2 h).ePercentage of WT parasi...