Maxisorp immuno plates (NUNC, Roskilde, Denmark) were coated with 5 μg/ml of the indicated kappa-5 variants and blocked with PBS/0.05% Tween. Then, plates were incubated with human infection sera diluted 1:100 and subsequently with alkaline phosphatase-labeled mouse anti-human IgE (Sigma). ...
Observations at higher magnifications indicated that MLG had a restricted distribution to the most inner and outer part of the cell wall (Fig. 5c). Figure 5 Unmasking MLG in stipes from Laminariales by immunofluorescence imaging. (a) Overview of MLG detection in cross-sections of stipes from ...
The β-chain of human complement component C8 exhibits a structural genetic polymorphism: using isoelectric focusing two major allotypes can be identified (C8B B ('basic') and C8B A ('acidic')). In the present report we describe a sequence polymorphism of the C8B gene (codon 63: AGA →...
Type III secretion systems (T3SSs) are bacterial membrane–embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member of theShi...
Mast cells are haematopoietic cells that arise from pluripotent precursors of the bone marrow. They play immunomodulatory roles in both health and disease. When appropriately activated, mast cells undergo degranulation, and preformed granule compounds are rapidly released into the surroundings. In many ca...
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G3BP1 and TIA, which are established SG components. As observed by others, a portion of TIA was also present in processing bodies (PBs, [54]). The oxidant-induced association of HspBP1 with SGs was not limited to HeLa cells; it also occurred in other cell lines that are derived from ...
HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor. FEBS Lett. 2002, 531, 339–342. [Google Scholar] [CrossRef] [Green Version] Zhao, T.; Hong, Y.; Yin, P.; Li, S.; Li, X.J. Differential HspBP1 expression accounts for the ...