Crosstalk between ion channels and small GTPases is critical during homeostasis and disease, but little is known about the structural underpinnings of these interactions. TRPV4 is a polymodal, calcium-permeable cation channel that has emerged as a potent
Overexpressing CCZ1-MON1A increased the active form of RAB7, enhanced autophagosome maturation, and promoted degradation of APP-CTFs, A尾 and P-tau in an autophagy-dependent manner in cells and a mouse AD model. Conclusions: Our data reveals that CCZ1-MON1A-RAB7 complex dysfunction is a ...
In the closed state, D531 (S2), Q550 (S3), D546 (S2-S3), and R594 (C-terminal half of S4; S4b) form a charged H-bond relay within the VSLD, while D743, R746 (TRP domain and Y439 (CD; HTHCD) connect the TRP domain and the CD (Fig. 3b, Nature Communications | (2023)...
O'Nuallain B, Freir DB, Nicoll AJ, Risse E, Ferguson N, Herron CE, Collinge J, Walsh DM (2010) Amyloid beta-protein dimers rapidly form stable synaptotoxic protofibrils. J Neurosci 30(43):14411–14419. doi:10.1523/JNEUROSCI.3537-10.2010 Article PubMed PubMed Central CAS Google Scholar...
To confirm that the appendage density corresponds to the CTD of Sec31, we analysed GUVs budded with a truncated form of Sec31 (encompassing residues 1 to 1114, referred to as Sec31-ΔCTD, Fig.3a). In cryo-tomograms of these tubules the outer coat was generally less ordered with respect...
In the F pilus, this loop, referred to as the “KNVK” loop, was hypothesized to form a contact with ssDNA during conjugative transfer (Paiva et al., 1992). The structures presented here locate this loop to the lumen of the pilus, suggesting that indeed the pilus serves as a conduit ...
In the closed state, D531 (S2), Q550 (S3), D546 (S2-S3), and R594 (C-terminal half of S4; S4b) form a charged H-bond relay within the VSLD, while D743, R746 (TRP domain and Y439 (CD; HTHCD) connect the TRP domain and the CD (Fig. 3b, Nature Communications | (2023)...
(E,F) The solvent accessible surface of AAV5 is colored according its electric potential as calculated using the APBS adaptive Poisson-Boltzmann solver [33]. Electro-negative surfaces (red), formed by charged side chains and polar backbone carbonyls, form pockets on AAV5 into which AAV5 R353...