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For immunoprecipitation of 3×FLAG-ZNF224 and c-MycPRMT5 complexes, anti-FLAG-M2-agarose beads and anti-c-Myc-agarose conjugate (20 μl of beads/mg of protein, Sigma) or IgG (5 μg/mg of protein, Sigma) were added to 1 mg of cell lysates, obtained with 50 mm Tris-HCl, pH 8.0...
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