We recorded coarse resolution images of the dextran in the early stages of meiosis. The acquired images were read and analysed using the Bioformats59 package within a MATLAB Compiler Runtime Environment. To eva
Half of the meiotic germ cells analyzed showed asynapsis, which was mostly extended or total. Discontinuity in the synaptonemal complex was seen in one third of the meiotic cells analyzed. An unusually small number of normal pachytene nuclei were found, all at early pachytene substages. ...
the male genetic program, but its modest amplitude at E11.5 and the high number of miR-17~92 putative targets genes that are upregulated in this pathway (40%) also argue for a complementary direct effect of miR-17~92 depletion, at least during the initial stages of sex determi- nation....
Dmrt1, sox9 and amh were observed to express in both early WT and the germ cell-depleted go- nads at 25 dph, but obvious down-regulated expression in WT gonads and significant up-regulated expression in the corresponding germ cell-depleted gonads were found at later ...
PGCs completed prophase of meiosis I but did not survive and form follicles. The same results were obtained when 12.5 dpc PGCs were aggregated with somatic cells from fetal ovaries of later developmental stages, competent to form follicles after kidney capsule transplantation orin vitroculture.25...
dynamically to IR: proteins involved in early stages of HR are quickly recruited to DSBs and promote expansion of the domain, and repair sites display a dramatic relocalization to outside heterochromatin, where they first recruit a protein (Rad51) required for strand invasion and completion of HR...
We have been able to maintain clonal cultures of Noctiluca scintillans throughout the whole life cycle and have revealed new details of various stages. In trophont populations, a small fraction of cells spontaneously transform into gametogenic cells, which undergo two successive nuclear divisions, ...
stages of 45 dph and 60 dph (Fig.4c-e). In addition,hsd3b,hsd11b2andcyp17a, were also revealed to have dynamic expression changes similar to that of testicular differentiation-related marker genes, in which obvious down-regulated expression in WT gonads and significant up-regulated expression...