Background Plastid genome content and protein sequence are highly conserved across land plants and their closest algal relatives. Parasitic plants, which obtain some or all of their nutrition through an attachment to a host plant, are often a striking exception. Heterotrophy can lead to ...
We also included additional copies of the genes fad1 and pdi1 from yeast, which have been shown to aid protein folding and enhance the production of flavin adenine dinucleotide (FAD), a key co-factor for EasE activity17. Following the modified YeastFab pipeline (Supplementary Fig. 1, ...
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We also identified candidate genes in the central pair, intraflagellar transport and dynein protein families that are associated with the formation of the spermatophore flagellum, which has been lost in all seed plants except ginkgo and cycads. The newly obtained Ginkgo genome provides new insights ...
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The Q. acutissima chloroplast genome encodes 136 genes, including 88 protein-coding genes, four ribosomal RNA genes, and 40 transfer RNA genes. In the repeat structure analysis, 31 forward and 22 inverted long repeats and 65 simple-sequence repeat loci were detected in the Q. acutissima cp ...
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The plastome contains 113 genes, of which 79 are protein-coding genes, 30 are tRNA genes, and 4 are rRNA genes. Sixteen genes contain one intron and two genes have two introns. A total of 45 simple sequence loci were identified from the genome. Phylogenetic analysis revealed that D. ...
The supernatant was then applied to a Ni-NTA column with a fast protein liquid chromatography (FPLC) system (GE Healthcare). The target protein was eluted using an imidazole gradient from 20 mM to 250 mM and the protein-containing fractions were collected. The protein solution was ...