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2 μL of the diluted cDNA product was employed as a template in a 20-μL reaction containing 1.0 μL of TaqMan miRNA Assay (Thermo Fisher Scientific), 7.67 μL of RNase-free water, and 10 μL of TaqMan™ Universal Master Mix II, with UNG (Thermo Fisher Scientific). The...
6 bacteria/ml saliva). The same procedure was employed to determine Lactobacilli in saliva using Dentocult LB stripes Low: <103 bacteria/ml saliva, Moderate: 103 –104 bacteria/ml saliva, High: >104 bacteria/ml saliva). (Orion Diagnostica, Espoo, Finland) [57...