The invention provides a preparation method for an efficient competent cell of escherichia coli. The step comprises the steps that escherichia coli is cultivated in a conventional liquid medium till an OD value is about 0.4, then cells are collected by centrifugation, and the competent cell is ...
Competent Cell Preparation and Transformation z TSS method for competent cell preparation Reagent:TSS solution 85% LB medium 10%PEG(wt/vol,MW8000)5%DMSO(vol/vol)50mM MgCl2(PH6.5)Autoclave or filter sterilize.Storage at 4℃for 2 weeks.1.Streak the cell stock on a LB plate.Incubate the ...
COMPETENT CELL PREPARATION & GROWTH SUPER-COMP 它是一种营养丰富的培养基,当使用一种简单的RbCa方法处理感受态宿主细胞时中,能显著增加细胞感受态。 产品信息: 货号 品名 规格 价格 113103014 10 X RB CA TXN SALTS 150 ml ￥1363.00 113103011 SUPER-COMP MEDIA (CAPSULES) 100 caps ￥1800.00 SOB 成分/L:20...
The optimal optical density (OD_ 600) range for competent cell preparation varied for each of the strains. The storage time of competent cells and its correlation to transformation efficiency were studied. Three critical alterations to previous methods were made, which were the changing of the ...
Competent Cell Preparation Kit是一种方便、高效、快速制备感受态细胞的试剂盒。使用本试剂盒制备的感受态细胞可以满足大多数实验的需要,并且适用于大多数常用的大肠杆菌,例如:E.coli DH5α、JM109、CJ236、HB101、MV1184、BMH71–18mutS等,转化效率均可以达到1×106 cfu/μg pUC19以上 (转化效率根据大肠杆菌细胞系...
本发明公开了一种高转化效率感受态细胞的制备方法,涉及微生物技术领域,解决现有技术操作不便,转化效率和得率低的问题. The present invention discloses a method for preparing high conversion efficiency competent cells of microorganisms relates to the field, to solve the inconvenience of the prior art operation...
英文名称:S. pombe Chemical Competent Cell Preparation Kit 纯度:99 % 产地:中国/上海 品牌:泽叶生物 包装规格:20次 货号:ZY100103A 别名:裂殖酵母化学感受墉细胞制备试剂盒 裂殖酵母化学感受态细胞制备试剂盒(S. pombe Chemical Competent Cell Preparati) ...
Heat-sealed plates for competent cell storage Be confident in your competent cell supply. FlexPlate-formatted competent cells are heat-sealed using a foil seal that does not delaminate even at very low temperatures and is easy to remove. Com...
1.一次制备,-80℃保存,多次使用,免去每次转化都要制备酿酒酵母感受态细胞。 2.操作简单,单溶液制备,除酵母培养的时间外,整个操作只需要30分钟。 3.主要用于酿酒酵母S.cerevisiae,也适用于其他多种实验用酵母菌株,包括S.pombe、C.albicans、Pichiapastoris等。
These methods involve treatment of cells with CaCl2 (1-5) or cobalt hexamine chloride (6). Agents such as polyethylene glycol (PEG) have also been shown to induce bacterial cell transformation (7). We now describe a new method for the preparation of competent bacterial cells that yields high...