For the titration process, 20.00 mL 0.0010 M Tyr-Gly solution was titrated with 0.020 mL HCl as titrant in each step and 0.050 mL each time with NaOH as titrant. After dropwise addition of the titrant solution, the pH value was recorded manually. The procedure was ended when the pH of ...
Synopsis: Buspirone hydrochloride ( HCl ) 1 is a new anxiolytic with a unique chemical structure. Its mechanism of action remains to be elucidated. Unlike the benzodiazepines, buspirone lacks hypnotic, anticonvulsant and muscle relaxan... KL Goa,A Ward - 《Drugs》 被引量: 217发表: 1986年 Asp...
the distinct role of the two COX isoforms in these processes still remains unclear. Using three characterized mouse lines where thePtgs1andPtgs2 genes substitute for one another, this study focused on the reproductive significance of their distinct roles and potential biological...
In further support of regulatory roles for the metal binding domains, the corresponding Cu+ chaperone in bacteria was found to to bypass the the ATPase metal-binding domain membrane entry site11. oMf othreeobvaecr,tebraisaeldPo1Bn-ttyhpee ATPase and instead crystal structure of a deliver Cu+ ...
As an additional proof that region −499 to −275 of the Epo promoter is a functional dioxin responsive enhancer, we compared the structure-activity relationship of this response to that observed using the CYP1A1 dioxin responsive enhancer. In this experiment, we examined the dose-response ...
Cell extracts were prepared in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 15 mM EDTA, 0.1% TritonX-100, 1 mM PMSF,) with protease inhibitor complex (Roche) on ice for 30 min. After protein concentration was detected using the Bradford method [22], 60 μg of cell extract ...
Sections were then washed 3 times for 5 min at RT in TNT (100 mM Tris-HCl pH9.5, 150 mM NaCl, 0.3% Triton X-100), and incubated at RT for 1 hr with Alexa 488-conjugated-tyramide in amplification buffer (1:100 dil.; Invitrogen, Carlsbad, CA) according to the Scientific Reports ...
Some organisms use a genetic mechanism to specify sex [1]. This often leads to degeneration of one homologue of the critical sex-determining chromosome. Consequently, these species evolve a mechanism to survive the otherwise lethal consequences of monosomy for a large chromosome in one sex. InDros...
The methods of the current invention compensate for variations in the structure of the solid phase reactants as well as other factors which can contribute to non-specific binding of components of the assays, thereby allowing more accurate determination of the amounts of analytes of interest in so...
In this report, human DPY-30-like protein was overexpressed and purified with the goal of structure determination. It was crystallized at 291 K in hanging drops by the vapour diffusion technique from a precipitant solution consisting of (NH4)2SO4 (1.5–2.0 M), Tris–HCl (0.1 M, pH 8.0...