Compartment Protein Extraction Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS 分析证书 数据表 Newsletters / Publications Key Applications: Protein Extraction 2145 1 kit Purchase on Sigma-Aldrich Recommended...
After DNA extraction with ethanol in phenol/chloroform, DNA concentrations were measured by spectrophotometry (260 nm) and cell numbers extrapolated from linear standard curves (data not shown). Conversely, protein concentrations were determined using the BCA protein assay kit (Pierce). Cell doubling ...
TotalRNA(1μg) was extracted from cultured cells using anRNA extractionkit (RNeasy Mini; Qiagen) and used to synthesize cDNA (ThermoScript PT-PCR System; Invitrogen). PCR amplification was performed in a total volume of 50μl containing 2 μl of cDNA, 2 mM MgCl2, 200 μM dNTP Mix, ...
The extraction of the RNA from the matrix based on collagen type I (renal tubules) was performed using the RNeasy Mini Kit (Qiagen, Hilden, Germany), according to manufacturer specifications. Each sample consisted of a pool from 3 renal tubule cultures simultaneously. Extraction from the agarose...
Real-Time PCR Quantitative mRNA Analysis RNA was extracted from PBS-perfused brain tissue using the RNeasy Lipid Tissue kit (Qiagen, Germany). Complementary DNA was synthesised using 1 mg of RNA through a reverse transcription reaction (Superscript II, Invitrogen, USA). tPA transcript levels were...
4. Reintam A, Parm P, Kitus R, Kern H, Starkopf J: Primary and secondary intra-abdominal hypertension – different impact on ICU outcome. Intensive Care Med 2008, 34:1624–1631. 5. Malbrain MLNG, De Laet IE: Intra-abdominal hypertension: evolving concepts. Crit Care ...
All mutant constructs were generated by site-directed (Quickchange kit, Stratagene) or PCR- based mutagenesis. In situ hybridization and immunofluorescence. For immunostaining analyses, 12.5 mm sections were generated from fixed embryos and slides were air-dried for 20 min. Slides were blocked in ...
Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 mMESSAGE mMACHINE T7 Kit Thermo Fisher Scientific Cat# AM1344 Amersham Megaprime DNA Labeling Systems GE Healthcare Cat# RPN1606 Nick Translation Mix Sigma-Aldrich Cat# 11745808910 PureLink RNA Mini kit Thermo Fisher Scientific Cat#...
SERCA activity was measured with a Ca2+-ATPase assay kit (Jiancheng Bioengineering Institute, Nanjing, China) and normalized to the protein concentration. This approach was the colorimetric measurement of Pi using the malachite green assay. An aliquot of extracted ER was added to the malachite ...
West Nile premembrane-envelope genetic vaccine encoded as a chimera containing the transmembrane and cytoplasmic domains of a lysosome-associated membrane protein: increased cellular concentration of the transgene product, targeting to the MHC II compartment, and enhanced neutralizing antibody response...