if possible, the mouse should be anesthetized. Since most imaging procedures do not incur pain, analgesia may not be required [33]. However, for invasive imaging techniques, the type of analgesia is determined according to
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Temporary immobilization for non-painful procedures For some non-painful procedures, the animal may require a higher level of sedation. This may be useful for imaging techniques that require temporary (~ 5 min) immobilization. It may be accomplished in mice by using a higher dose of acepromazin...
Biology Department, Vancouver Island University, Nanaimo, British Columbia, Canada Colin A. Chapman Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi’an, China Colin A. Chapman School of Life Sciences, University of KwaZulu-Natal, Scottsville, South Africa Colin A. Chapman Labo...
Computational biologyis a branch of biology that uses computational and mathematical approaches to model and analyzebiological systems. It involves the development and application ofalgorithms,statistical methods, andcomputational modelsto understandbiological processes, predict outcomes, and gain insights from ...
We thank Minh Hien Le for her assistance in the lab. Author information Authors and Affiliations Department of Behavioural Biology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria Carina Siutz, Marc Pluch & Eva Millesi Department of Integrative Biology and Evolution, University of ...
The development of genome-wide gene expression profiling technologies, using microarrays first and sequencing since, has brought the analysis of gene expression into the realm of systems biology. There exist a variety of techniques such as arrays, SAGE, CAGE, and RNA-seq that allow for different ...
Evaluation of growth conditions and DNA extraction techniques used in the molecular analysis of dermatophytes. Journal of Applied Microbiology. 2017; 122(5):1368-1379. 19. Zamani Z, Bahar M, Jacques MA, Lak MR, Akhavan A. Genetic diversity of the common bacterial blight pathogen of bean, ...
(TTP Labtech Inc.). Sequencing adapters were based on the iTru protocol86, where short universal adapter stubs are ligated first followed by addition of sample-specific barcoded sequences in a subsequent PCR step. Amplified and barcoded libraries were quantified by the PicoGreen assay and ...
(to directly activate B cells) or, alternatively, using α-CD3/CD28 (CD3/28) (to define the influence of the T-cell compartment on B cells). We then used droplet-based techniques32to create a transcriptomic census of different immune cells of the CVID-discordant twin pair and performed ...