Briefly, cells were homogenized in lysis buffer (TRIS pH 7.6 50 mM, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA and 1% SDS) and samples were heated at 95 ◦C for 15 min, sonicated and pre-cleared by centrifugation for 10 min at 10,000 g. Proteins were resolved in SDS-PAGE, transferred...