In addition to equilibrium binding affinity the kinetic rate constants, for antigen association with an antibody binding site and their dissociation, are of particular interest. They are important to current discussions about what would be the most effective properties for targeted delivery of ...
Then, the pO2 of the chamber was suddenly dropped to 21%, triggering a relaxation towards a new equilibrium.43 Since the oxygen content in the perovskite influences the electronic conductivity, we used the conductivity transient between the initial and the final states to investigate the ORR ...
The enzyme concentra- tions were chosen so that the reactions were linear during the 220-s incubation period, and all reactions were carried out in triplicate. Blank wells lacked the enzyme but included the kinase buffer, the substrate, and ATP. The plates were covered, and the reactions were...
Reactions were incubated for 10 min to reach equilibrium, and 10-μl aliquots were loaded onto 5% native acrylamide gels (29:1 ratio of acrylamide to bis-acrylamide) in 0.33× TBE (1× TBE is 90 mm Tris borate, 1mm EDTA, pH ∼8.3). Gels were run in 0.33× TBE at 200 V, dried...
3. “Global” Thermodynamic and Kinetic Parameters for Folding/Unfolding Reactions Obtained from 1D High-Pressure NMR Spectroscopy Typically, 1D HP-NMR can be used for steady-state measurements, recorded when the ratio between folded/unfolded protein populations has reached equilibrium after a pressure ...
where C0 and Ce are the initial and equilibrium liquid-phase concentrations of sodium oleate, respectively (mg/L), V is the volume of the solution (L), and W is the weight of the magnesite used (g). The measured equilibrium data (Ce, qe) were fitted to the Freundlich Equation (2) ...