Combining existing drug therapy is essential in developing new therapeutic agents in disease prevention and treatment. In preclinical investigations, combined effect of certain known drugs has been well established in treating extensive human diseases. A
For pharmaceutical and clinical applications, human hepatocytes must be introduced into liver organoids. To this end, we co-cultured human reprogramming hepatocytes (hCLiP), which can robustly differentiate to form functional hepatocytes in vitro21, with mouse cholangiocytes. When hCLiP were cultured...