if yes what is the way to regenerae the column which have <5µm particle size and what may be the procedure. Logged JGK Chemist Full Member Posts: 738 Mole Snacks: +66/-19 Gender: Re: Column regeneration « Reply #1 on: January 18, 2011, 02:50:08 PM » Some modern HPLC...
Process for the regeneration of a strong cation exchange chromatography column after elution of compounds of interest comprising the following steps in this order: - eluting adsorbed polypeptides from the column with an aqueous buffered solution comprising sodium chloride at a concentration of at least ...
Equilibrate the column set by performing two blank injections (DI water) under the test chromatogram conditions, including the column regeneration and re-equilibration steps. Once the columns are equilibrated, inject a system suitability standard such as the column's QAR standard, to establish the ...
Equilibrate the column set by performing two blank injections (DI water) under the test chromatogram conditions, including the column regeneration and re- equilibration steps. Once the columns are equilibrated, inject a system suitability standard such as the column's QAR standard, to establish the ...
2. Use the tubing and fittings (supplied with the column regeneration valve) to connect the OUT port of the column selection valve to the detector inlet. 3. Route the line through the door of the column heater (or column heater/cooler). Ensure the line is routed and dressed through the ...
effluent of the displacement column. By working at 60°C the mobile phase flow-rate could be increased to an extent where the time required for a single analysis was reduced to 20 s. Including column regeneration, an analytical frequency of 2 min−1could be maintained. As the flow-rates ...
(The regeneration of columns depends on this property.) However, when the cations lie well to the left in the above series it is often advantageous to use a complex-forming species to facilitate removal. For example, iron can be displaced from ion-exchange columns by passage of sodium ...
The analysis time of the fully automated procedure is 45 min. The selectivity of the system is demonstrated with direct injections of spiked urine and milk samples. The regeneration and the stability of the immuno precolumn and the general applicability of non-selective desorption for on-line ...
RegenerationProcedurefortheAlltech ® Anion/RColumn Pumpthefollowingsolventsthroughthecolumnsequentiallyat 1mL/min: 1.100mL50mMdisodiumEDTA(adjustedtopH10.0 withsodiumhydroxide) 2.100mLdeionizedwater 3.100mL50mMsulfuricacidin50%acetonitrile:water 4.100mLdeionizedwater ...
This regeneration procedure clearly shows that the chromium can be removed in a small fraction of the waste volume, after the sulphate has been removed and co-incidentally at the beginning of the nitrate removal stage of the process (FIG. 3). In FIG. 3, Cr (mgl−1) and NO3, SO4(mg...