High throughput HPLC — Alternating column regeneration with the Agilent 1100 Series valve solutionsDriven by the demand to analyze and purify large numbers of samples in drug discovery, researchers nowadays do not only need highthroughput analytical HPLC equipment but also ask for fully automated and...
Regeneration method of reversed-phase packing column of HPLC (high performance liquid chromatograph)The invention discloses a regeneration method of a reversed-phase packing column of HPLC (high performance liquid chromatograph). The regeneration method comprises steps as follows: (1), the reversed-...
Re: Column regeneration «Reply #1 on:January 18, 2011, 02:50:08 PM » Some modern HPLC columns do not have a truly symmetrical design which may lead to problems if they are reversed. My copy of "Practical HPLC" (V. K Meyer) suggests the following for regenerating C18, C8, Phenyl...
TheColumn Swapper™Alternating Column Regeneration System TheLC Sample Peak Recycler™Analytical Recycle HPLC System TheLC-MS Watchdog™Source Contamination Minimizer The2D Multidimensional Column Switching Systemfor LC x LC HPLC Expert HPLC Method Development Consulting (esp. Chiral): ...
Equilibrate the column set by performing two blank injections (DI water) under the test chromatogram conditions, including the column regeneration and re-equilibration steps. Once the columns are equilibrated, inject a system suitability standard such as the column's QAR standard, to establish the ...
Please contact ProZyme for advice on filters suitable for HPLC sample preparation. Column Cleaning, Regeneration, and Storage After heavy use, your GlycoSep R column may become contaminated with strongly adsorbed sample constituents, which will lead to a loss in column performance. To clean the ...
TheLC Spiderling™line of Automated HPLC Column Selectors TheColumn Swapper™Alternating Column Regeneration System TheLC Sample Peak Recycler™Analytical Recycle HPLC System TheLC-MS Watchdog™Source Contamination Minimizer The2D Multidimensional Column Switching Systemfor LC x LC HPLC ...
In such cases, follow these steps for column cleaning and regeneration. If these procedures will not solve the problem, then we recommend that you use new column First replace the mobile phase to shipping solvent. Then inject 4 – 5 mL of following solvents ((1) – (4)) with running ...
(5) In order to save costs, it can be reused after activation and regeneration. The regeneration method is: repeat steps (1) and (2) for regeneration. Generally, a RP column can be regenerated 5 times. Precautions: (1) The IC-Ag column is not renewable. ...
Cleaning and Regeneration If the backpressure increases or a shift in selectivity is observed, use the following recommended column wash procedure. 30 column volumes of deionised water 30 column volumes of 0.5 M NaCl 30 column volumes of deionised water An initial washing with deionised water is ...