The limit of quantification (LOQ) of the alternative method was around 20 genomic equivalents per PCR reaction, i.e. 2.3 log 10 live Campylobacter per ml of sample. The alternative method passed a relative trueness study, confirming the robustness against different meat rinses, and displayed ...
The PCR amplification of the NC10 phylum pmoA gene was performed using primer pairs A189_b/cmo682 and cmo182/ cmo568, as previously described51. Briefly, the PCR mixtures (25 μL) contained 1 μL of template DNA, 1 μL of each primer, 9.5 μL of RNAase-...
D PCR products from genomic DNA using toxin-specific primers show that the intra-colony genetic variation is at least in part due to toxin allelic variants. Genomic was extracted from individual ants belonging to the same colony as those used for our transcriptome and main proteomic data. DNA ...
(a) RT-PCR analysis of CSF1 and CSF1R mRNAs in A549-luc cells; human breast cancer cell lines, MDA MB-231 and BT20, were used as controls. Total RNA was isolated from cells and RT-PCR was carried out using human CSF1, CSF1R, or actin (control) primer sets. A549 cells express ...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that caused the outbreak of the coronavirus disease 2019 (COVID-19). The COVID-19 outbreak has led to millions of deaths and economic losses globally. Vaccina
(A) Real-time PCR. (B and C) Western blot. Normal neutrophils (1 ϫ 106 cells/mL) from adults and neonates were isolated at 0 h. Real-time PCR was used to examine the fold increase in XIAP (A) in RNA from adults and neonates. (B) Western blotting was also used. These blots ...
Mice were sacrificed 6 h later, and mRNA and protein levels of inflammatory markers were measured from brain tissue via real-time PCR (normalized to the housekeeping gene GAPDH) and MSD® Multi-Spot Assay, respectively (Fig. 1c–f). As expected, LPS treatment robustly increased RNA levels ...
Real-time reverse transcription polymerase chain reaction (RT-PCR). Sorted PDPN+ and PDPN− cells were suspended in 1 mL of TRIzol reagent (Life Technologies, Carlsbad, CA, USA) and stored at −80 °C. Total RNA was purified from thawed samples through standard techniques, ...
(100 ng/mL). Real-time PCR was performed as previously described85. Accordingly, total cellular RNA was isolated using the RNeasy Plus Kit with the removal of genomic DNA according to the manufacturer’s instructions (Qiagen, Valencia, CA). Nucleic acid concentrations were determined by NanoDrop...
Levels of miR-155 and its candi- date target genes expression were analyzed by real-time PCR. U6 or GAPDH levels were used as endogenous control of miR-155 or mRNA levels, respectively. Levels of the miR155 and all analyzed target genes were normalized to the respective levels detected in ...