Transforming growth factor-alpha and epidermal growth factor receptor in colonic mucosa in active and inactive inflammatory bowel disease. Growth Factors 2000; 18: 79–91. Article CAS PubMed Google Scholar Oikonomou Ka, Kapsoritakis AN, Kapsoritaki AI, Manolakis AC, Tsiopoulos FD, Germenis AE...
In short, genomic DNA was prepared from 10–15 mg of proximal colon mucosa using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA). The DNA (1 µg) was modified via bisulfite treatment using the EpiTect Bisulfite Modification Kit (Qiagen) followed by PCR amplification using 1...