Colon cancer cell lines classified by the molecular pathways CIN, MSI and CIMP, and mutation status of cancer critical genes Cell line MSI status CIMP panel 1 CIMP panel 2 CIN KRAS BRAF PIK3CA PTEN TP53 CO-115 DLD-1 HCT-116 HCT-15 LoVo LS-174T RKO SW48 TC71 Caco-2 COLO 320 EB ...
In a series of 11 microsatellite stable (MSS) and 9 microsatellite unstable (MSI) colon cancer cell lines and primary colon carcinomas (25 MSS and 28 MSI) with known ploidy stem line and APC, KRAS, and TP53 mutation status, we analyzed the promoter methylation of the following genes: h...
2010CRC cell line; LIM1215, murine mast cells and human urine.Better understanding of the physiological role of exosomes in colon cancer biology.Immuno-affinity capture of exosomes (A33 antibody) proteins were separated by 1D-SDS-PAGE followed by in gel tryptic digestion.LC-MS/MS (LTQ-Orbitrap...
31 and used the newest version of the CMSclassifier package with the Random Forest prediction method for CMS classification of colon cancer cell lines10. We then chose 14 cell lines covering all CMS subtypes. The selected cell lines and their genetic status are shown in Table 1. Importantly, ...
Rate of dissemination and prognosis in early and advanced stage colorectal cancer based on microsatellite instability status: systematic review and meta-an... MSI-H CRC was associated with an overall survival benefit with a lower rate of dissemination. Survival benefit was clearly evident in both st...
[160] The NCCN guidelines note that although high MSI status or MMR deficiency associated with the BRAF V600E mutation is usually due to epigenetic mechanisms and is not inherited, it does not rule out Lynch syndrome; approximately 1% of cancers with BRAF V600E mutations (and loss of MLH1)...
Lowe SW, Bodis S, McClatchey A et al.: p53 status and the efficacy of cancer therapy in vivo. Science 266: 807–810, 1994 Google Scholar Hargest R, Williamson R: Expression of the APC gene after transfection into a colonic cancer cell line. Gut 37: 826–829, 1995 Google Scholar ...
8.2.3.1.1Genomic instability: an evolving hallmark of colon cancer Most colon cancers are characterized by genomic instability. This is the result of genomic alterations during cell division. Damage to multiple genes that control cell division and tumor suppressors results in the development of cancer...
We isolated clonal cell populations from the HGUE-C-1 primary culture after 15 passages in culture (non-tumour cells have already undergone senescence at that point). We then analysed isolated clonal populations of HGUE-C-1 cells to determine MSI status (results shown in Figure 1) and their...
(MSI-H) status are distinguished by their heightened production of tumor antigens, resulting in effective T cell recognition, activation and response to ICIs. However, only a small fraction (approximately 10–15%) of CRC patients exhibit dMMR/MSI-H status, while most (85–90%) are ...