FIGURE 3.Relative chloramphenicol acetyltransferase (CAT) activity of construct containing thecis-acting regulatory regions of the collagen type II gene. Note the high level of CAT expression in chondrocytes contrasting with the other cell types. ...
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(a) Each of the six individual α-chains contains an N-terminal 7S domain, a central collagenous domain with multiple interruptions, and a C-terminal noncollagenous domain (NC1). (b) Three α-chains assemble in a defined fashion to form the three types of protomers. All three are found ...
All proteins that have a structure based on three helix polypeptide chains belonging to the collagen family, being identified 26 types until now[10, 11]. The unique structure of collagen is responsible for its fibrous nature that is very hard to degrade[3]. Until now, the molecule has been...
Available Media Types o 10x Concentrates: Ham’s F-12 medium, MEM with Hanks’ balanced salt solution, DF medium (DME:F-12 = 1:1), and Medium 199. o 5x Concentrates: DME medium and RPMI-1640. Reconstitution Buffer Specifically formulated for collagen-gel cultures. ...
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6c). These results suggest that host cells had synthesized a collagenous matrix enriched in the same collagen types as are found in the normal cornea. Overall, there were differences in the amounts of individual α chains, β-dimers and γ trimers of collagen between CLP-PEG-MPC and CLP-...
However, head vasculature did not show any autofluorescence and was strongly stained with ColVI antibodies (Supplementary Fig. S5a). Since in situ hybridization showed the presence of col6a1 transcripts in various cell types (Fig. 2a and Supplementary Fig. S3b), we further examined ColVI ...
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Of these individuals, 16 were selected for screening because they had clinical findings compatible with the diagnosis of EDS type I or II (the classical types in the revised nomenclature) (Beighton et al. 1998). The clinical findings are summarized in table 1. All samples were obtained with ...