InnoCyte™ ECM Cell Adhesion Assay, Collagen Type IV A convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent...
These 96-well plates are coated with PureCol® collagen, the standard among collagen products for purity (>99.9% collagen content), consistency and reproducibility. Collagen coated plates are ideal for cell culture and various cell assays.
The inhibition exerted by Col-IV on T production in response to hCG was also observed when cells were stimulated with 8Bromo-cAMP. In addition, conversion of steroid precursors to T in LC cultured on uncoated and Col-IV coated plates was similar. On the other hand, we detected an increase...
Human Collagen IV Mouse Collagen IV Vialed Formats(Cat. No./Qty.) 354245 | 0.25 mg 354233 | 1 mg 356233 | 10.0 mg (10 x 1 mg) Applications A ubiquitous component of the basement membrane. The sheet-like matrix is found in close proximity to epithelial, muscle, and nerve cells. ...
(9, 10). This non-specific binding is especially pronounced when sample dilution is low. Therefore, when using our ELISA Grade Collagen to assay human sera, we highly recommend using non-collagen coated plates to determine the background signal for each individual sample you plan to assay. ...
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV (COL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% Linearity...
This product contains freshly hepatocytes plated in 12-well collagen coated plates with matrigel overlay at 24 hours and cultured for 48 hours with ≥75% confluency prior to shipping. Note: Fresh hepatocyte products are available only in the United States. To get notified on isolation schedule and...
IV[α1(IV)]2[α2(IV)]; other forms are also knownBasement membraneAlport Syndrome: characterized by nephritis and sensorineural deafness V[α1(V)]3Skin, Placenta, Blood vessels, Chorion uterusEDS types I & II [(α1(V)]2[α2(V)] ...
T-cell responses are measured by T-cell proliferation assay, and antibody by enzyme-linked immunosorbent assay (ELISA) with peptide-coated plates. We next tested whether pCol(a30-40) was nephritogenic. Rats that received a single immunization with pCol(a30-40) could be divided into two disti...
1 μg of collagen I (Sigma, C3867), collagen IV (Sigma, CC076), collagen VI (Corning, 354261), laminin (Sigma, L2020), protective antigen (List Biological Labs,174 A) and BSA in 100 μl PBS were coated for 2 h at room temperature on 96-well tissue culture plates. Exces...