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The quantitative real-time polymerase chain reaction (qPCR) analysis was performed using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a Kapa SYBR Fast qPCR kit (Kapa Biosystems, Woburn, MA, USA). The qPCR reaction consisted of an initial ...
WTS hiPSC cells were seeded as single cell suspension on human recombinant laminin 521 (Biolamina) coated CellBindⓇ 24 well plates (Corning) at densities from 11 000 cells/cm2 to 52 000 cells/cm2 in Essential 8™ Flex medium (Gibco). Cells were incubated for 24 h at +37 °C in ...
longstandmg RA mvolvmg both her knees and ankles, wlth her left knee bemg warm, mIldly erythematous, tender to palpatlOn, and demonstratmg decreased arc of flexlOn Radl- ographs of the left knee showed mcreased bony and jomt destruc- tIon, whlch had progressed over the precedmg year...
Flex NG ion source (Thermo Scientific, Bremen, Germany). A full MS scan was followed by data-dependent MS2 scans in a 3 s cycle time. Precursor ions selected for MS2 were excluded for subsequent MS2 scans for 20 s. The resolution for the full scan mode was set as 120,000 (atm/z200...