1980 Metaphase chromosome associations: colcemid distorts the pattern. Cytogenet. Cell Genet. 27, 98–110.Bodman TC, Flehinger BJ, Rohlf FJ (1980) Metaphase chromosome associations: colcemid distorts the pattern. Cytogenet Cell Genet 27:98–110...
Colcemid binds to the protein tubulin and arrest cells in metaphase for karyotyping assays. Colcemid incuces cell apoptosis and can be used for cancer research[2]. 分子量 371.43 Formula C21H25NO5 CAS 号 477-30-5 运输条件 Room temperature in continental US; may vary elsewhere. 储存方式...
The proportion of metaphase-II (MII) oocytes after 30 hr of IVM was higher in control (79.6%) than in COL0-22 oocytes (61.7%); overall nuclear progression to the MII stage was not influenced by colcemid treatment by the end of the IVM period (93.8, 86.7, 86.8, and 84.8% for ...
Effects of metaphase inhibitors (colcemid, colchicine, vinblastine) on mouse and human embryonic, fibroblast-like cells growing on glass and on an oriented substrate (fish scale) were studied. All three inhibitors caused similar changes in the form of interphase cells and inhibited their directional ...
When metaphase cells were reseeded with 40 ng/ml of colcemid, they delayed anchorage to a dish; 6 hr was required for complete adhesion (in the absence of colcemid only 1 hr was required). When reseeded metaphase cells were exposed to 40 ng/ml of colcemid for 5 hr followed by its ...
Behavior of sperm nuclei in intact and bisected metaphase II mouse oocytes fertilized in the presence of colcemid. Gamete Res 1988; 20: 365-376.Borsuk E, Mańka R (1988) Behavior of sperm nuclei in intact and bisected metaphase II mouse oocytes fertilized in the presence of colcemid. Gamete...
Further studies revealed that the G2/M arrest in IMR-32 correlates with both increased bubR1 nuclear localization and metaphase arrest. The siRNA knockdown of bubR1 has decreased tumor cell response to geldanamycin suggesting Hsp90-dependent regulation of bubR1. The combination treatment also showed...
At metaphase, the amount of tubulin assembled into spindle microtubules is relatively constant; the rate of tubulin association equals the rate of dissocia... ED Salmon,M Mckeel,T Hays - 《Journal of Cell Biology》 被引量: 447发表: 1984年 THE MECHANISM OF COLCHICINE INHIBITION OF MITOSIS. ...
By using Colcemid or vinblastine sulfate, HeLa cells were arrested in metaphase and centrifuged on the colloidal silica density gradient. The blocked ... DA Wolff,H Pertoft - 《Journal of Cell Biology》 被引量: 39发表: 1972年 Separation of HeLa cells by colloidal silica density gradient centri...
(1) Treatment of metaphase-arrested spindles ofPectinariaoocytes with Colcemid (N-desacetyl-N-methylcolchicine, a colchicine derivative with greater effectiveness and less toxicity), reduces spindle length and birefringence reversibly, confirming the action of colchicines reported onChaetopterus. (2)D2O...