Visualization of specific collagen-producing cells by Col1-GFP transgenic mice revealed novel type I collagen-producing cells other than fibroblasts in systemic organs/tissues.Type I collagenPodocytesLeydig cellsTransgenic mouseType I collagen is one of the most abundant proteins in mammals and plays ...
Recently transgenic mice in which green fluorescent protein (GFP) expression is under the control of the rat 3.6 (pOBCol3.6GFPtpz) and 2.3 (pOBCol2.3GFPemd) Col1a1 promoter fragments were generated. Our analysis of these GFP-expressing transgenic mice shows that the 2.3-kb promoter fragment ...
By using the coronal portion of dental pulps isolated from postnatal transgenic mice our observations also provide direct evidence that the dental pulp contains progenitor/stem cells capable of giving rise to a new generation of odontoblast-like cells, as well as osteoblast-like cells. 展开 ...
Mice were generated in which a Col2-GFP transgene serves as a reporter for the chondrocyte lineage and for chondrogenesis in live embryos and newborn pups. Cells actively engaged in chondrogenesis were identified by confocal optical sectioning within their native environments in embryos and in thick...
Transgenic mice were generated that harbor a Col2-GFP reporter that marks chondrocytes and their immediate precursors during skeletal development. Cells engaged in chondrogenesis were identified by conventional fluorescence microscopy and confocal optical sectioning within their native environments in live ...
Our previous analysis of transgenic mice in which 2.3-kb fragment of the rat Col1a1 promoter drives the expression of GFP (pOBCol2.3GFP transgenic animals) showed that 2.3-GFP transgene is expressed at low levels in functional odontoblasts and at high levels in terminally differentiated odontoblas...
We have examined and compared the effects of various differentiation-inducing media on mineralization, cell morphology and expression of pOBCol3.6GFP (3.6-GFP) in primary dental pulp cultures derived from 3.6-GFP transgenic mice. Our results show that media containing ascorbic acid only could not ...
Cellular colocalization of the chondrocyte-derived tomato marker and a 2.3Col1-GFP osteoblast specific marker in femur sections of Col10a1-Cre;2.3Col1-GFP;ROSA-tdTomato triple transgenic mice.Xin, Z...
Transgenic mice were generated that harbor a Col2-GFP reporter that marks chondrocytes and their immediate precursors during skeletal development. Cells engaged in chondrogenesis were identified by conventional fluorescence microscopy and confocal optical sectioning within their native environments in live ...
Cellular colocalization of the chondrocyte-derived tomato marker and the osteoblast-specific 2.3Col1-GFP marker in femurs of tamoxifen treated Agc1-CreERT2;2.3Col1-GFP;ROSA-tdTomato triple tr...