加之在Rosetta的框架下进行骨架生成,可以直接进行更加复杂的侧链结构优化,甚至是构建特异性识别的氢键网络,设计可通过离子的疏水空腔等,从而实现跨膜蛋白、逻辑门调控元件、pH敏感型蛋白以及离子通道等功能蛋白的设计(更多细节请参考Baker实验室已发表文献) 一. 计算原理与细节 1.1 Crick公式与参数 α螺旋
Here, we report that a coiled-coil domain of Boi2 cooperates with the adjacent PH domain to confer Boi2's bud-cortex localization and major function in cell growth. The PH domain portion of the PH-CC bi-domain interacts with the Rho GTPases Cdc42 and Rho3 and both interactions are ...
除此之外,生物 体中还有许多调节性的二聚化作用,如病毒膜蛋白HA中的coiled coil结构域参与 低pH诱导的病毒与细胞膜的融合;细菌的rlpA转录抑制因子正常状态下二聚体 和单体处于平衡,温度升高诱导coiled coil-*聚体减少,解除转录抑制而激活某 些蛋白的表达 【1ll;裂殖酵母分裂过程中,My02蛋白C端coiled coil区一...
pH, and interactions with surfaces or biomolecules influence these interactions and thus, the structure and stability of the coiled coils [18,21–23]. The fine balance between attractive and repulsive interactions that can regulate folding and assembly of coiled coils make these molecules highly inter...
低pH 诱导的病毒与细胞膜的融合;细菌的rlpA 转录抑制因子正常状态下二聚体 和单体处于平衡,温度升高诱导 coiled coil-*聚体减少,解除转录抑制而激活某 些蛋白的表达【1ll;裂殖酵母分裂过程中,My02 蛋白 C 端 coiled coil 区一个位点 的 磷酸化对于其定位到细胞骨架动力环是必须的【121。
(50 mM Tris at pH 8.0, 150 mM NaCl, 250 mM imidazole). Fractions containing the protein of interest were merged and injected into a size exclusion column (HiLoad 26/600 Superdex 200 pg, GE Healthcare, IL, USA) and separated at 2.6 mL/min (20 mM Tris at pH 7.5, ...
1170Accesses Metrics Abstract Targeted therapy of chronic myeloid leukemia (CML) is currently based on small-molecule inhibitors that directly bind the tyrosine kinase domain of BCR-ABL1. This strategy has generally been successful, but is subject to drug resistance because of point mutations in the...
Kinesin-1 neck-linker crystals were grown at 20 °C via vapor diffusion from a 1:1 mixture of 15 mg/ml protein solution and 20% (w/v) methoxy polyethylene glycol (MEPEG) 5000, 100 mm Li2SO4, 100 mm MES/acetate, pH 5.5, 10 mmγ-caprolactone. Kinesin-1 crystals appeared spontaneously...
dynamic('PhiCA', 283, 27), # 默认为g位上的Interface angle] def get_buff_total_energy(ampal_object): return budeff.get_internal_energy(ampal_object).total_energy # 运行优化 for index, func in enumerate([ev_opts.GA, ev_opts.PSO, ev_opts.DE, ev_opts.CMAES]): opt = func(...
The protein IMX313T was purified on a Hi Trap SP FF 5 ml column equilibrated with 50 mM sodium phosphate pH 7.4. The protein was eluted in a 0 to 1M NaCl gradient. Fractions containing the protein IMX313T were pooled, dialyzed against 1× PBS and applied to a Hi Load 26/60 Supe...