Samples (0.3 g of freeze-dried sample) were placed into Pyrex culture tubes to which 0.3 mL of the C13:0 internal standard (0.5 mg of C13:0/mL of MeOH), 0.56 mL of 10 M KOH in water, and 4.24 mL of MeOH were added. The tubes were incubated in a 55 °C water bath for 1.5...
Each sample was analyzed in triplicate. Statistical analysis. The expression levels of the miRNA and mRNA were calculated using the 2–ΔΔCT method, in which the value represents the n-fold change in the test samples relative to their corre- sponding control. The data are expressed as ...
Glass beads with lithium tetraborate were employed to minimize the preferential orientation of phyllosilicates. The detection limit for major elements is 0.01 wt.%. Loss on ignition (LOI) was determined using 0.5 g of powdered sample, first dried at 110 °C and then heated to 1000 °C for 1...