(2006) Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system. J. Biosci. Bioeng. 102, 511-517....
By analysing natural sequences, we show that there is a clear bias for triplets corresponding to stop codons near the 5′- and 3′-splice sites in the introns of various clades.Similar content being viewed by others Detecting macroevolutionary genotype–phenotype associations using error-corrected ...
Four-base codons for assigning the positions of nonnatural amino acids were also selected. Mutated streptavidin mRNAs that contained different four-base codons were prepared and added to the insect cell-free system in the presence of various tRNAs possessing the corresponding four-base anticodons. ...
As a result, the substantial criterion for the judgment of the plausibility of any hypothesis concerned and the consensus basis for different concepts in this field have been covered. This may explain the long term controversies among various theories in the field of the origin of genetic codons....
The different cur- rent phenotypes of full length and N-terminal deletion channels allowed us to determine the absence or presence of the N-terminus in various Shaker mutants expressed in Xenopus oocytes using the cut-open voltage clamp technique27. Results Functional channels are translated despite...
Various studies have demonstrated that specific polymorphisms, such as S127 and M142, extend the incubation period of the disease but confer less resistance than do codons 146 and 222 [22,23,24,25]. On the other hand, H154 has been identified as a potential risk factor for atypical scrap...
Two reviews (2, 14) summarise in tabulated form those references that report synonymous mutations associated with aberrant splicing, thus leading to various human diseases, for example optic atrophy (15) , McArdle disease (16) , X-linked hydrocephalus (17) , thrombasthe-nia (18) , glycine ...
The standard genetic code is reputedly optimized to minimize mutation impacts on various criteria, from amino acid polarity (Ardell, 1998; Freeland and Hurst, 1998), protein folding (Guilloux and Jestin, 2012; Caudron and Jestin, 2012; Seligmann and Warthi, 2017), overlap coding (Popov et al...
Molecular biologists have discovered that the amino acid sequence in a protein synthesized is based on sets of three nucleotide bases in messenger RNA called codons. In theory, how many different codons in RNA can be formed by vario...
First, there were no differences between the various cathepsin L genotypes when ES cells undergo differentiation and a cathepsin L-dependent histone H3 clipping was not observed. Second, the epiblast-derived embryo showed no morphological aberrations. In summary, these data exclude crucial functions of...