Genetic code expansion technologies supplement the natural codon repertoire with assignable variants in vivo, but are often limited by heterologous translational components and low suppression efficiencies. Here, we explore engineeredEscherichia colitRNAs supporting quadruplet codon translation by first developing...
These conditions are obvi-ously not identical in cases when the translation proceeds with more or less uniform speed along the polypeptide chain, and when the ribosome stalls at certain codons, thus leaving time for the already fi nished part of the chain to fold into a stable structure and ...
Immunoblot analysis with the m6A antibody (top) shows that levels of m6A in poly(A) RNA (left) and poly(A) tail-depleted RNA (right) are comparable. Removal of poly(A) tails was confirmed using 3′RACE and RT-PCR to detect β-actin; no product is detected in the tail-depleted ...