E. coli codon usage master table.Julius, B. Lucks
When codon usage in the organism of origin is starkly different from that of the organism used for expression, for example when a human gene is expressed in E. coli, codon optimization (i.e. replacement of rare codons with more frequent synonymous codons) can to an increase in expression ...
coli.24 The transfer RNAs (tRNAs) corresponding to rare codons are only found in minor populations in E. coli cells and coding sequences containing several rare codons (especially in iterative arrangements) are likely to become poorly expressed. Besides premature termination the misincorporation of ...
Codon usage in bacteria: correlation with gene expressivity. Nucleic Acids Res. 10, 7055–7074 (1982). Article CAS PubMed PubMed Central Google Scholar Xia, X. How optimized is the translational machinery in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae? Genetics 149, 37–...
especially in highly expressed genes. For example,E. colivery rarely uses two of the six codons that specify arginine (AGA and AGG). This nonrandom distribution, calledcodon usage, correlates with the abundance of the corresponding tRNA, in cases studied. This kind of information is used in ...
was significantly different from those of E. coli and yeast genome. 籽粒苋NAD-ME基因与大肠杆菌、酵母的基因组密码子偏好性有较大差异。 The underlined nucleotides are the first upstream in-frame stop codon and the two putative polyadenylation signals, respectively. 带下划线的核苷酸序列分别为第一个...
(Assaad and Signer, 1990). Therefore, cloning a gene that has been codon-optimized for plants downstream of theCaMV35Spromoter could potentially lead to instability in bacterial cells if the gene has a large number of rareE. colicodons. Hence, codon usage may need to be considered when ...
3,55,56,57,58,59 The connection between synonymous codon usage and transcript stability has now been expanded to numerous prokaryotic and eukaryotic organisms, including E. coli, S. pombe, Neurospora, Xenopus, zebrafish, Drosophila, mice, and humans, suggesting that this phenomenon is deeply ...
Asterisks indicate termination co- dons, The SSU rRNA gene is underlined assuming its nucleotide se- quence has similarity to that of E. coli. The seven tRNA genes are also underlined and their anticodons are dotted. The abbreviation of protein-genes is the same as in Fig. 1. This region...
L Holm Codon usage and gene expression Nucleic Acids Res, 14 (1986), pp. 3075-3087 86176786 CrossrefView in ScopusGoogle Scholar [59] AJ Makoff, MD Oxer, MA Romanos, NF Fairweather, S Ballantine Expression of tetanus toxin fragment C in E coli: high level expression by removing rare c...