D. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques 30, 892–897 (2001). Article PubMed CAS Google Scholar Hansen, K. D., Brenner, S. E. & Dudoit, S. Biases in Illumina transcriptome sequencing caused by random hexamer ...
Using this wiring diagram, we generated a computational network model to predict neural coding properties at cellular resolution across three brain regions. When relating the wiring diagram to function, a common approach starts by extracting rules of synaptic connectivity between cell types and then app...
The PCR was carried out using the DOP-PCR library, derived from flow-sorted B chromosomes of the Siberian roe deer, as template. Parallel amplification was conducted with bovine and total roe deer genomic DNAs. The PCR products of appropriate size resulting from the B chromosome-specific ...
www.intechopen.com Molecular Cloning, Characterization, Expression Analysis and Chromosomal Localization of the Gene Coding for the Porcine αIIb Subunit of the αIIbβ3 Integrin Platelet Receptor 115 Primers Primer sequences 5'- 3' Template, Product Tm localization (5'-5') size (bp) (ºC)...
In the PCR amplification reaction, if the single strand of the amplified template needs to be folded back, the structure formed by folding back is likely to compete with the combination of primer and template. Thus, high-efficiency reading cannot be realized [15]. Figure 1. Secondary structure...
For qRT-PCR, 1 ng of template cDNA and 1xTB Green II (Takara #RR820) were added to each well of a 96-well plate (Axygen, Union City, CA, USA, #PCR-96-FSC) and covered with an optical adhesive film (Applied Biosystems ABI, Waltham, MA, USA, #4311971) prior to execution on a...
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It allows regenerating the PSNR template file to be filed by proponents by re-computing frame PSNR from provided decoded YUV files. The second tool proposed is an Excel sheet with advanced customization functionalities to compute and graph various objective metrics (e.g. BD-rate, BD-PSNR, …)...
pFL-Ddx3x_wt_30UTR and pFL-Crb1_wt_30UTR vectors, containing full-length 30-UTRs of mouse Ddx3x (NM_010028.3) or Crb1 (NM_133239.2) mRNAs, respectively, were generated with RT–PCR using retinal cDNA as a template. The amplified fragments were cloned into the XbaI site present ...
After incubation for 4 h at 37 °C, template DNA was degraded by TURBO DNase (Ambion) treatment. The non-labeled competitors were in vitro transcribed using the MEGAscript T7 kit (Life Technologies) according to the manufacturer’s instructions. ATTO-680 labeled probes and unlabeled ...