We note that the PLAR pipeline does not permit lncRNAs that overlap introns of protein-coding genes on the same strand (due to the difficulty in their annotation). Of the lncRNA transcripts, 1,460 did not overlap those annotated in the GENECODE release 24/Ensembl release 84. When grouping ...
their length is significantly different from other experimentally defined sRNA loci (Two-sided Wilcoxon rank sum test: W = 1,547,014, p = 1.02e−27). In addition, inspecting the distribution of the proportion of
The circular + strand (Red) of viroid RNA is copied into the–strand (Blue) by the RCR, which is linearized by the host RNases, and the linear RNA is then used as the template for the synthesis of the + strand. Finally, the host ligases circularize the viroid RNAs. As shown in ...