Coding Strand vs Template Strand Lesson Summary Frequently Asked Questions What is the difference between coding strand and template strand? The coding strand of DNA is the strand that codes for the gene of interest. The template strand is complementary to this and can be transcribed to produce...
A group of linear lncRNAs with peculiar features is represented by NATs. The development of high-throughput sequencing has shed light on this class of RNA molecules that are transcribed from the opposite DNA strand. They originate from bidirectional promoters shared with the corresponding sense transcr...
communication, and repetitive and stereotyped interests. ASDs represent a spectrum of conditions of varying severity, and may or may not be accompanied by intellectual disability, epilepsy, or other features; underscoring this phenotypic heterogeneity, mutations in genes associated with classical monogenic...
RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# 10161310 High Sensitivity D1000 ScreenTape Agilent Cat# 5067- 5582 High Sensitivity RNA ScreenTape Agilent Cat# 5067-5579 Qubit RNA HS Assay Invitrogen Cat# Q32852 Luminaris HiGreen qPCR Master Mix, low ROX Thermo Fisher Scientific Cat# ...
ncRNAs can also be broadly categorized by genomic properties such as 1) sense or antisense transcripts, when the ncRNA transcript co-localizes with exons of another transcript on the same or the opposite strand, respectively, 2) bidirectional, when the expression of the ncRNA and of a neighborin...
The simplest are antisense RNAs transcribed from the opposite DNA strand as the mRNA that they regulate. In some, but not all cases, binding of the sRNA to its mRNA target requires the RNA chaperone Hfq (Møller et al., 2002, Soper and Woodson, 2008, Zhang et al., 2002). Thus, in...
Such vector constructs must also include a packaging signal, long terminal repeats (LTRS) or portion thereof, and positive and negative strand primer binding sites appropriate to the retrovirus used (if these are not already present in the retroviral vector). Optionally, the recombinant lentiviral ...
Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs, we id
SENCR is transcribed from the AS strand and partially overlaps an intron of the neighbour friend leukaemia virus integration 1 (FLI1) protein-coding gene. Although SENCR and FLI1 are co-expressed in various tissues, KD studies showed that they do not affect each other's expression. SENCR ...
Then, the first-strand cDNA was diluted with DNase/RNase-free water to the concentration of 1 ng/μL. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) reaction was performed using 1 ng of cDNA template per reaction with PowerUp SYBR Green Master Mix (Thermo Fisher ...