Protein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site-specifically lactylated proteins in living cells, ...
Protein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site-sp
For a few specific proteins, single-molecule approaches have been used to accurately monitor translation elongation rates and related co-translational protein-folding processes. In some cases, it was clearly shown that the slow-down of translation elongation is crucial to facilitate proper co-translatio...
Genetic code expansion (GCE) allows site-specific incorporation of noncanonical amino acid (ncAA) in proteins, which enables tight control of messenger RNA translation by requiring the presence of a ncAA to overcome premature termination resulting from an ‘ectopic’ codon. Briefly, GCE can be achie...
Wenshe R. Liu has provided two protein engineering work, with one focusing on pyrrolysyl-tRNA synthetase engineering to improve the ncAA incorporation efficiency,22 and the other on providing the structural basis for high incorporation efficiency of o-chlorophenylalanine into proteins.23 Through ...
The coupled functioning of that marvelous pair of macromolecules, nucleic acids and proteins, has been the basis for the outstanding success of living systems on Earth. The nucleic acids act primarily in the storage, protection and transmission of the genetic information. In accordance with this ...
You must have a WebView2 runtime installed on your machine with a minimum version of 111. Download the WebView2 Runtime You must install the Remote Tools for Microsoft Edge from the Microsoft Store: Remote Tools for Microsoft Edge - Microsoft Store Apps 1. Configure your Windows device ...
(ReLU) activation functions. In this paper, we present an in-depth analysis of methods for maximizing the sparsity of the activations in a trained neural network, and show that, when coupled with an efficient sparse-input convolution algorithm, we can leverage this sparsity for significant ...
a role in the evolution of new proteins and in the development of gene families. For example, mutations that introduce new codons for a particular amino acid can result in the formation of new genes that encode slightly different proteins, leading to the evolution of new functions or ...
35x faster (with almost no decrease in BLEU) over the state-of-the-art inference implementation. Machine Translation NMT +1 8 Paper Code TILGAN: Transformer-based Implicit Latent GAN for Diverse and Coherent