A. Design of a bacterial host for site-specific incorporation of p-bromophenylalanine into recombinant proteins. J. Am. Chem. Soc. 128, 11778–11783 (2006). Article CAS PubMed Google Scholar Park, H.-S. et al.
Next, we compared NATS system with Tet-On system25, a commonly used transcriptional turn-on switch, for inducer exposure time and response time. As shown in Fig.2c, only 1-min exposure to OmeY was sufficient to effectively induce the production of SEAP, whereas the Tet-On system needed at...
For a few specific proteins, single-molecule approaches have been used to accurately monitor translation elongation rates and related co-translational protein-folding processes. In some cases, it was clearly shown that the slow-down of translation elongation is crucial to facilitate proper co-translatio...
Protein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site-sp
The coupled functioning of that marvelous pair of macromolecules, nucleic acids and proteins, has been the basis for the outstanding success of living systems on Earth. The nucleic acids act primarily in the storage, protection and transmission of the genetic information. In accordance with this ...
Wenshe R. Liu has provided two protein engineering work, with one focusing on pyrrolysyl-tRNA synthetase engineering to improve the ncAA incorporation efficiency,22 and the other on providing the structural basis for high incorporation efficiency of o-chlorophenylalanine into proteins.23 Through ...
J. (1961) General nature of the genetic code for proteins, Nature, 192, 1227-1232. Article CAS PubMed Google Scholar Nirenberg, M., and Leder, P. (1964) RNA codewords and protein synthesis. The effect of trinucleotides upon the binding of sRNA to ribosomes, Science, 145, 1399-1407...
(ReLU) activation functions. In this paper, we present an in-depth analysis of methods for maximizing the sparsity of the activations in a trained neural network, and show that, when coupled with an efficient sparse-input convolution algorithm, we can leverage this sparsity for significant ...
In this section, you create a function app and related resources in your Azure subscription. Many of the resource creation decisions are made for you based on default behaviors. For more control over the created resources, you must insteadcreate your function app with advanced options. ...
35x faster (with almost no decrease in BLEU) over the state-of-the-art inference implementation. Machine Translation NMT +1 8 Paper Code TILGAN: Transformer-based Implicit Latent GAN for Diverse and Coherent Text Generation 1 code implementation • Findings (ACL) 2021 • Shizhe Diao,...