cleaned wet glass slides at room temperature. Chromosomes were stained with 5% Giemsa (karyoMax, Thermo Fisher) in GURR buffer (Gibco). At least 30 metaphase spreads were captured and analyzed for karyotyping using an Axioplan2 microscope (Zeiss) and Ikaros (MetaSystems) software. Flow cytometr...
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Amplification of (ACAG)n and (AATC)n was found on the W chromosome of tiger snakes (N. scutatus), while (AGAT)n was also observed in the Japanese striped snake (E. quadrivirgata), and the tiger keelback snake (R. tigrinus) [26] (Fig. 5). However, the sets of microsatellite repe...
The machine comes with the upgraded controller, latest software, High Speed Intelligent Scanners and variable power high pulse energy lasers. Mike Sparidaens of Excellon said, “Cirexx is realizing the benefits from the enhanced technology of this next generation Cobra-II laser. As our product ...
Double-stranded consensus oligonucleotides (5′-AGTTGAGGGGACTTTCCCAGGC-3′; Promega Corp., Madison, WI) containing the DNA-binding site for NF-κB were end-labeled with [γ-32P]ATP. The extracted nuclear proteins (10 µg) were incubated with 0.1 ng of 32P-labeled DNA for 15 min at ...
Image analysis was performed with ImageJ and Imaris software. Movies were first contrast enhanced in ImageJ, and a walking average of four frames was taken using the kymograph plug-in for ImageJ. These steps were performed to improve the accuracy of automated particle recognition performed in sub...
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