Herein, we have investigated the function of the ADF-H family member coactosin-like 1 (COTL1) in the regulation of actin dynamics at the T cell immune synapse (IS). We initially identified COTL1 in a genetic screen to identify novel regulators of T cell activation, and subsequently found...
Coactosin-like protein (CLP, or Cotl1), is an F-actin-binding protein, whose role in cancer is largely unknown. Here we show that CLP/Cotl1 is highly expressed in a rat epithelial breast cancer cell line (FE1.3) compared with its mesenchymal counterpart (FE1.2). Knockdown of CLP/Cotl1...
residuesintheactivesite. Detailedstructure analysis showedthe catalytic histidineandserinetobe present both intwoconformations,whichprovides a deepinsie#t intothe catalytic mechanismfor acetyl transfer. hCLP-Humancoactosin-like protein isanactinfilament bindingprotein butdoesnot bindtO globular actin.It...
(f-actin), we determined whether clp could physically link 5lo to actin filaments. however, no f-actin-clp·5lo ternary complex was observed. in contrast, 5lo appeared to compete with f-actin for the binding of clp. moreover, 5lo was found to interfere with actin polymerization. our ...
2006. Binding model of human coactosin-like protein with filament actin revealed by mutagenesis. Biochim Biophys Acta 1764:1688-1700.Dai H, Huang W, Xu J, Yao B, Xiong S, Ding H, Tang Y, Liu H, Wu J, Shi Y: Binding model of human coactosin-like protein with filament actin ...
Localization of the actin binding protein, coactosin that expressed in the developing chick ciliary ganglionInduced pluripotent stem cellsParkinson's diseasetransplantationdopaminergic neuronspositron emission tomographyBefore induced pluripotent stem cells (iPSCs) can be used to treat neurologic diseases, ...
actin-binding proteinCoactosin was first isolated from Dictyostelium discoideum and, as reported, human coactosin-like protein (CLP) was identified in a yeast two-hybrid screen using 5-lipoxygenase (5LO) as a bait. A mouse CLP (mCLP) cDNA clone was identified among EMBL/GenBank EST ...
Upregulation of an actin binding protein, Coactosin during maturation of parvalbumin-positive interneurons in mouse visual cortexdoi:10.1016/j.neures.2011.07.565XubinHouandSayakaSugiyamaSDOSNeuroscience Research