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An entry vector for Gateway Cloning (Thermo Fisher Scientific) provided by A. Herzig encoding a histone gene unit (His-GU) was used as a template to simultaneously mutate H3K18 and H4K8 to either H3R18 and H4R8 or to H3Q18 and H4Q8 (see Supplementary Table 5 for primers used)....
for relevant information Please note that not all kits are provided with Certificate of Analysis. Electronic Certificate of Analysis is initially not applicable for small volume products, ImmunoCAP Rapid, ImmunoCAP ISAC, EliA or Varlisa products. This Search and Download function may be unavailable for...
Coverslips for Actin labeling were incubated inside a 1:40 dilution in PBS of rhodamine-phalloidin stock remedy (Thermo Fisher Scientific) for 20 min. To detect presences of enzymes, cells were incubated over night at 4 C with main antibodies diluted in PBS supplemented with 3% bovine serum ...
and reverse primer pairs (Additional file1: Table S4) using the SuperScript III First-Strand System for RT-PCR Kit (Thermo Fisher Scientific,https://www.thermofisher.com). The cDNAs were cloned into pENTR-D Topo and then into pDEST17 Vector (Thermofisher Scientific) by LR recombination ...
Sections were then mounted with Permount (Thermo Fisher Scientific, Canada) and allowed to dry overnight before imaging. Dendritic spine density was assessed by counting spines found on secondary and tertiary dendrites of neurons in the dentate gyrus and the CA1 of the hippocampus on a light ...
2-Methylbutyric acid, acetic acid, chloroform, and methanol were purchased from Thermo Fisher Scientific. CoA and butyryl-CoA were purchased from Avanti. [U-13C]acetyl-CoA was from Sigma-Millipore. [13C2]Acetyl-ACP standard was made as described (15). [d7]Isobutyric acid and [d9]iso...
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(62248, ThermoFisher Scientific) during the blocking step. Cells were washed three times in PBS and prolong diamond antifade mountant (P36965, ThermoFisher Scientific) was used to mount the coverslips. Images were acquired using Structured Illumination Microscopy (Nikon SIM), and analyzed in ...